Protocol for micropropagation of Balanites aegyptiaca (L.) Del. using seeds was established. Seeds obtained from mature trees were sterilized and cultured into Murashige and Skoog (MS) medium. In-vitro raised seedlings nodal portion was used as explant. Explant culture were established in solid basal Murashige and skoog (MS) medium supplemented with different concentration and combination of kinetine puriss (6-furfuryl amino purine) 1, 3, 6 mg/l and indole-3-acetic acid (IAA) 0.1 mg/l for shoot proliferation and multiplication. The culture were maintained at 25±2°C under 7/5/12 hours/day i.e. 7 h/day photoperiod from cool white fluorescent lamps, 5 h/day dimlight, 12 h/day in dark. Result shows that shoot multiplication required 3 mg/l of kinetine puriss for sufficient growth.
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